KINETIC STUDIES OF β-GLUCOSIDASE PRODUCED FROM ASPERGILLUS NIGER NRRL 567

Abstract

β-glucosidase was produced from Aspergillus niger NRRL 567 grown on 2% corn stover under continuous shaking for 96 hours. Maximal enzyme activity was obtained at pH 3.5 and temperature 30 ^oC. Kinetic investigations revealed that according to Lineweaver-Burk plot, Woolf Augustisson-Hofstee plot and Hanes-Woolf plot, Bgl had K_M 0.45, 0.39 and 0.40% and Vmax 1.01, 0.93 and 0.95 mmol mL^-1min^-1., respectively. Energy of activation E_a for the enzyme was found to be 19.0 kJK^-1mol^.-1, whereas rate of reaction was increased to 13.3 after every 10 ^oC rise in temperature. Conclusively, small K_M value of the enzyme showed a large affinity of the enzyme with substrate. Therefore, the enzyme was found good catalytic agent for the bioconversion of waste material into glucose and biomass protein.